Organic Chemistry

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I need help with a mix of microbiology/biochem/pharma. I did an experiment where I made mimics of TB antibiotics in a lab. I need to understand the structural activity relationship of the compound or stereochemistry. I tested this on a bacteria as a kirby bauer disk test. The variations I made didn't inhibit the bacteria. I need to know why not and what's the mechanism of action [MOA] of my compound/drug molecule. I have an idea regarding the MOA, I just need verification.
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